Study of the action of peripheral blood T-lymphocytes on renal cell carcinoma cells in model systems

Background. The introduction of immunotherapy based on immune checkpoint inhibitors has significantly improve the effectiveness kidney cancer treatment. Nevertheless, not all patients respond to such treatment and there are no reliable predictive markers. Therefore, development a model system for assessing cellular response tumor seems be an urgent task. Aim. Development assess T-cell was focus this study. Materials methods. Primary cell culture peripheral blood fraction were obtained under standard sterile conditions. activation perform via anti-CD3 anti-CD28 antibodies. index assessed using RTCA xCELLigence biosensor technology (ACEA Biosciences, USA). Results. Tumor T-cells from same patient cultured together growth rate population. Measurements taken at 30-minute intervals. duration observation 24 hours. It been shown that non-activated do affect proliferative properties cells. On contrary, activated suppressed cells, which associated with increase in proportion Т-cells carrying HLA-DR receptor (CD3+HLA–DR+) because activation. Tumor-specific activity can lead three consequences: lack effect, partial suppression properties, complete death In latter case, absence cells determined by flow cytometry. Conclusion. developed approach makes it possible evaluate cytotoxic T relation particular patient. advantage method is measurement carried out presence inhibitors. proposed may useful evaluating regimen within framework personalized therapy.